Effects of Non-Essential Amino Acids on Knee Joint Conditions in Adults: A Randomised, Double-Blind, Placebo-Controlled Trial

Nutrients. 2022 Sep 2;14(17):3628. doi: 10.3390/nu14173628.


Joint problems impair performance during exercise and daily activities and influence quality of life. The present study aimed to examine the effects of a combination of six non-essential amino acids (6AA) on joint conditions in an adult population. A total of 50 participants aged between 20 and 64 years with joint discomfort but no diagnosed joint disorder were randomly and blindly assigned to a control or 6AA group. The 6AA group took 12 g of the non-essential amino acid formulation orally (4 g three times a day) and the control group took equivalent doses of a placebo. Each group maintained the daily dose for 12 weeks. Primary outcome measures were evaluated with the visual analogue scale (VAS), the Japanese Knee Osteoarthritis Measure (JKOM), and the Japanese Orthopaedic Association score (JOA). These tests were taken before the experiment began at 4 weeks and 12 weeks after the intervention. The results of the VAS indicated that 6AA improved joint pain, discomfort, and stiffness both during a resting state and during normal activity. Participants' scores on the JKOM and JOA also showed significant improvements in the group that had taken the 6AA supplement. These results demonstrate that 6AA improves symptoms of joint problems, such as pain, discomfort, stiffness, and difficulty in performing daily activities after 4 weeks of daily consumption.

Keywords: JKOM; JOA; VAS; amino acids; joint.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Amino Acids / pharmacology
  • Arthralgia / drug therapy
  • Double-Blind Method
  • Humans
  • Knee Joint
  • Middle Aged
  • Osteoarthritis, Knee* / drug therapy
  • Quality of Life*
  • Treatment Outcome
  • Young Adult


  • Amino Acids

Grants and funding

This research received no external funding.