The primary structure of human secretogranin I (chromogranin B): comparison with chromogranin A reveals homologous terminal domains and a large intervening variable region

EMBO J. 1987 May;6(5):1203-11.

Abstract

We have determined and analyzed the primary structure of human secretogranin I (chromogranin B), a tyrosine-sulfated secretory protein found in a wide variety of peptidergic endocrine cells. A 2.5-kb cDNA clone, hybridizing to an mRNA of similar length, was isolated from a cDNA library of human pheochromocytoma. The identity of the clone was established by comparison of its deduced amino acid sequence with N-terminal and several internal secretogranin I sequences as well as by immunoprecipitation of the protein produced by in vitro transcription-translation of the cloned cDNA. Secretogranin I is a 657 amino acid long polypeptide of 76 kd and is preceded by a cleaved N-terminal signal peptide of 20 residues. Comparison of the predicted amino acid sequence of human secretogranin I with that of bovine chromogranin A reveals significant homologies near the N termini and at the C termini. The N-terminal homologous domains contain the only two cysteine residues of both proteins and form disulfide-stabilized loop structures. The sequences between the homologous terminal domains in both proteins differ but are characterized by a remarkable hydrophilicity, an abundance of acidic amino acids and potential dibasic cleavage sites for the generation of smaller, perhaps hormone-like, peptides.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Gland Neoplasms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromogranin A
  • Chromogranin B
  • Chromogranins / genetics*
  • Chromogranins / isolation & purification
  • Cloning, Molecular
  • DNA / metabolism
  • Genetic Variation*
  • Humans
  • Nerve Tissue Proteins / genetics*
  • Nucleic Acid Hybridization
  • Pheochromocytoma
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid

Substances

  • CHGA protein, human
  • CHGB protein, human
  • Chromogranin A
  • Chromogranin B
  • Chromogranins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/Y00064