Transcription of H-2 and Qa genes in embryonic and adult mice

EMBO J. 1987 May;6(5):1265-71.

Abstract

We have isolated cDNAs for three class I genes from an 8.5-day C57Bl/6 mouse embryo cDNA library. Two of these cDNAs encode the classical transplantation antigens, H-2Kb and H-2Db. The third is a novelly spliced form of a Qa region gene, Q9, which lacks several exons including that encoding the transmembrane domain. Since this mRNA is detected on membrane-bound polysomes it may encode a secreted protein. Transcripts for these three genes and the pseudo-allele of Q9, Q7, were present at very low levels in the 8.5-day and 14.5-day embryo and in the 2-day neonate, compared to most adult tissues. Q10, the liver-specific secreted class I gene product in adults, is preferentially expressed in yolk sac and in fetal liver. We found no embryo-specific class I gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / genetics*
  • Cloning, Molecular
  • DNA / analysis
  • Embryo, Mammalian
  • Exons
  • Genes
  • H-2 Antigens / genetics*
  • Histocompatibility Antigens Class I*
  • Introns
  • L Cells / immunology
  • Major Histocompatibility Complex*
  • Mice
  • Mice, Inbred C57BL
  • Tissue Distribution
  • Transcription, Genetic*

Substances

  • Antigens, Surface
  • H-2 Antigens
  • Histocompatibility Antigens Class I
  • Q surface antigens
  • DNA