Neuronal nicotinic acetylcholine receptor beta-subunit is coded for by the cDNA clone alpha 4

FEBS Lett. 1987 Jul 27;219(2):459-63. doi: 10.1016/0014-5793(87)80272-7.


Acetylcholine receptors (AChRs) with high affinity for nicotine but no affinity for alpha-bungarotoxin, which have been purified from rat and chicken brains by immuno-affinity chromatography, consist of two types of subunits, alpha and beta. The beta-subunits form the ACh binding sites. Putative nicotinic AChR subunit cDNAs alpha 3 and alpha 4 have been identified by screening cDNA libraries prepared from rat PC12 cells and rat brain with cDNA probes encoding the mouse muscle AChR alpha-subunit. Here we determine the amino-terminal amino acid sequence of the rat brain AChR beta-subunit by protein microsequencing to be the same as amino acid residues 27-43 of the protein which could be coded by alpha 4. Further, we present evidence consistent with a subunit stoichiometry of alpha 3 beta 2 for this neuronal nicotinic AChR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism*
  • Bungarotoxins / metabolism
  • Chickens
  • Cloning, Molecular*
  • DNA / metabolism*
  • Genes*
  • Macromolecular Substances
  • Neurons / metabolism*
  • Nicotine / metabolism
  • Rats
  • Receptors, Nicotinic / genetics*
  • Receptors, Nicotinic / isolation & purification
  • Receptors, Nicotinic / metabolism


  • Bungarotoxins
  • Macromolecular Substances
  • Receptors, Nicotinic
  • Nicotine
  • DNA