Dihydrotestosterone was implanted directly into the brain of ovariectomized all-female Cnemidophorus uniparens. Only implants located in the anterior hypothalamus-preoptic area (AH-POA) induced male-like pseudocopulatory behavior. Implants in other brain regions, including the ventromedial hypothalamus (VMH), failed to elicit mounting and intromission behavior. Implants in the VMH also failed to elicit female-like receptive behavior. Radioimmunoassay revealed no significant difference in circulating levels of androgen between the responding and the nonresponding animals. These findings support previous studies implicating the AH-POA as the major integrative area for male-typical mounting and intromission behavior.