Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes

J Biol Chem. 1987 Jul 25;262(21):10035-8.


Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced directly. The membranes are not chemically activated or pretreated with Polybrene before usage. The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs. The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • Lactoglobulins / analysis*
  • Membranes
  • Methods
  • Myoglobin / analysis*
  • Polyvinyls
  • Rosaniline Dyes


  • Lactoglobulins
  • Myoglobin
  • Polyvinyls
  • Rosaniline Dyes
  • polyvinylidene fluoride
  • Coomassie blue