Molecular characterization of transesterification activity of novel lipase family I.1

Biosci Rep. 2022 Oct 28;42(10):BSR20220654. doi: 10.1042/BSR20220654.


Lipase's thermostability and organic solvent tolerance are two crucial properties that enable it to function as a biocatalyst. The present study examined the characteristics of two recombinant thermostable lipases (Lk2, Lk3) based on transesterification activity. Conversion of C12-C18 methyl ester with paranitrophenol was investigated in various organic solvent. Both lipases exhibited activity on difference carbon chain length (C12 - C18, C18:1, C18:2) of substrates. The activity of Lk2 was higher in each of substrate compared with that of Lk3. Experimental findings showed that the best substrates for Lk2 and Lk3 are C18:1 and C18:2 respectively, in agreement with the computational analysis. The activity of both enzymes prefers on nonpolar solvent. On nonpolar solvent the enzymes are able to keep its native folding shown by the value of radius gyration, solvent-enzyme interaction and orientation of triad catalytic residues. Lk3 appeared to be more thermostable, with maximum activity at 55°C. The presence of Fe3+ increased the activity of Lk2 and Lk3. However, the activity of both enzymes were dramatically decreased by the present of Ca2+ despite of the enzymes belong to family I.1 lipase known as calcium dependent enzyme. Molecular analysis on His loop of Lk2 and Lk3 on the present of Ca2+ showed that there were shifting on the orientation of catalytic triad residues. All the data suggest that Lk2 and Lk3 are novel lipase on the family I.1 and both lipase available as a biocatalyst candidate.

Keywords: Lipase recombinant; Solvent tolerance; Thermostability; Transesterification; molecular docking; molecular interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium*
  • Carbon
  • Enzyme Stability
  • Esters
  • Lipase* / genetics
  • Lipase* / metabolism
  • Solvents / chemistry


  • Esters
  • Solvents
  • Carbon
  • Lipase
  • Calcium