ALS mutations in the TIA-1 prion-like domain trigger highly condensed pathogenic structures

Proc Natl Acad Sci U S A. 2022 Sep 20;119(38):e2122523119. doi: 10.1073/pnas.2122523119. Epub 2022 Sep 16.


T cell intracellular antigen-1 (TIA-1) plays a central role in stress granule (SG) formation by self-assembly via the prion-like domain (PLD). In the TIA-1 PLD, amino acid mutations associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) or Welander distal myopathy (WDM), have been identified. However, how these mutations affect PLD self-assembly properties has remained elusive. In this study, we uncovered the implicit pathogenic structures caused by the mutations. NMR analysis indicated that the dynamic structures of the PLD are synergistically determined by the physicochemical properties of amino acids in units of five residues. Molecular dynamics simulations and three-dimensional electron crystallography, together with biochemical assays, revealed that the WDM mutation E384K attenuated the sticky properties, whereas the ALS mutations P362L and A381T enhanced the self-assembly by inducing β-sheet interactions and highly condensed assembly, respectively. These results suggest that the P362L and A381T mutations increase the likelihood of irreversible amyloid fibrillization after phase-separated droplet formation, and this process may lead to pathogenicity.

Keywords: intrinsically disordered protein regions; liquid–liquid phase separation; neurodegenerative diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids* / chemistry
  • Amino Acids* / genetics
  • Amyotrophic Lateral Sclerosis* / genetics
  • Amyotrophic Lateral Sclerosis* / metabolism
  • Distal Myopathies / genetics
  • Distal Myopathies / metabolism
  • Humans
  • Mutation
  • Prions* / chemistry
  • Protein Aggregation, Pathological* / genetics
  • Protein Conformation, beta-Strand / genetics
  • Protein Domains / genetics
  • T-Cell Intracellular Antigen-1* / chemistry
  • T-Cell Intracellular Antigen-1* / genetics


  • Amino Acids
  • Prions
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human