ZmDWF1 regulates leaf angle in maize

Plant Sci. 2022 Dec;325:111459. doi: 10.1016/j.plantsci.2022.111459. Epub 2022 Sep 13.

Abstract

Leaf angle (LA) is a critical agronomic trait enhancing grain yield under high-density planting in maize. A number of researches have been conducted in recent years to investigate the quantitative trait loci/genes responsible for LA variation, while only a few genes were identified through map-based cloning. Here we cloned the ZmDWF1 gene, which was previously reported to encode Δ24-sterol reductase in the brassinosteroids (BRs) biosynthesis pathway. Overexpression of ZmDWF1 resulted in enlarged LA, indicating that ZmDWF1 is a positive regulator of LA in maize. To reveal the regulatory framework of ZmDWF1, we conducted RNA-Sequencing and yeast-two hybrid (Y2H) screening analysis. RNA-Sequencing analyzing results indicate ZmDWF1 mainly affected expression level of genes involved in cell wall associated metabolism and hormone metabolism including BR, gibberellin, and auxin. Y2H screening with Bi-FC assay confirmed three proteins (ZmPP2C-1, ZmROF1, and ZmTWD1) interacting with ZmDWF1. We revealed a new regulatory network of ZmDWF1 gene in controlling plant architecture in maize.

Keywords: Leaf angle; Maize; Regulatory network; ZmDWF1.

MeSH terms

  • Edible Grain / metabolism
  • Gene Expression Regulation, Plant
  • Phenotype
  • Plant Leaves* / genetics
  • Plant Leaves* / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • RNA
  • Zea mays* / genetics
  • Zea mays* / metabolism

Substances

  • RNA
  • Plant Proteins