Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Sci Rep. 2022 Sep 21;12(1):15763. doi: 10.1038/s41598-022-19964-1.

Abstract

Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for infectious diseases in animal models of human disease and in the veterinary context. To expand the knowledge of serum N-glycan profiles in important mammalian model systems, in this study, we combined MALDI-TOF-MS analysis and HILIC-UPLC profiling of released N-glycans together with glycosidase treatments to characterize the glycan structures present in rhesus macaque serum. We used this baseline to monitor changes in serum N-glycans during infection with Brugia malayi, a parasitic nematode of humans responsible for lymphatic filariasis, in a longitudinal cohort of infected rhesus macaques. Alterations of the HILIC-UPLC profile, notably of abundant structures, became evident as early as 5 weeks post-infection. Given its prominent role in the immune response, contribution of immunoglobulin G to serum N-glycans was investigated. Finally, comparison with similar N-glycan profiling performed during infection with the dog heartworm Dirofilaria immitis suggests that many changes observed in rhesus macaque serum N-glycans are specific for lymphatic filariasis.

MeSH terms

  • Animals
  • Biomarkers
  • Brugia malayi*
  • Dirofilaria immitis* / physiology
  • Dogs
  • Elephantiasis, Filarial* / parasitology
  • Glycoside Hydrolases
  • Humans
  • Immunoglobulin G
  • Macaca mulatta
  • Mammals
  • Polysaccharides

Substances

  • Biomarkers
  • Immunoglobulin G
  • Polysaccharides
  • Glycoside Hydrolases