Fluctuations in Intestinal Microbiota Following Ingestion of Natto Powder Containing Bacillus subtilis var. natto SONOMONO Spores: Considerations Using a Large-Scale Intestinal Microflora Database

Nutrients. 2022 Sep 16;14(18):3839. doi: 10.3390/nu14183839.


Improving the intestinal microbiota using probiotics, prebiotics, and synbiotics has attracted attention as a method of disease prevention and treatment. This is the first study to discuss the effects of food intake on the intestinal microbiota using a large Japanese intestinal microbiota database. Here, as a case study, we determined changes in the intestinal microbiota caused by ingestion of a processed natto food containing B. subtilisvar. natto SONOMONO spores, SONOMONO NATTO POWDER CAPSULESTM, by analyzing 16S rRNA sequence data generated using next-generation sequencing techniques. The results showed that the relative abundance of Bifidobacterium and Blautia as well as the relative abundance of Bifidobacterium were increased in males and females in the ingesting group, respectively. Additionally, the effects of SONOMONO NATTO POWDER CAPSULESTM intake on Bifidobacterium and Blautia abundance depended on the relative abundance of Bifidobacterium at baseline. Finally, analysis of a large Japanese intestinal microbiota database suggested that the bacterial genera that fluctuated with the ingestion of SONOMONO NATTO POWDER CAPSULESTM may be associated with lifestyle-related diseases such as diabetes.

Keywords: 16S rRNA; Bacillus subtilis var. natto SONOMONO; Bifidobacterium; Blautia; fermentation; gut microbiota; intestinal microbiota; natto.

MeSH terms

  • Bacillus subtilis
  • Bifidobacterium / genetics
  • Eating
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Male
  • Powders
  • Probiotics* / pharmacology
  • RNA, Ribosomal, 16S / genetics
  • Spores, Bacterial


  • Powders
  • RNA, Ribosomal, 16S

Grants and funding

This research received no external funding.