CD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections

Viruses. 2022 Sep 16;14(9):2056. doi: 10.3390/v14092056.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) has a strict cell tropism. In addition to the primary alveolar macrophages, PRRSV is strictly cytotropic to African green monkey kidney cells, such as MARC-145 cells; however, MARC-145 cells are not infected by most NADC30-like and NADC34-like PRRSV strains. The essential scavenger receptor CD163 has been proved to mediate productive infection of PRRSV in various non-permissive cell lines. In this study, we systematically tested the porcine CD163 stably expressing 3D4/21 cells for infections with various PRRSV strains. The results showed that the porcine CD163-expressing macrophages support the infections of PRRSV2 of lineages 1, 5, and 8, as evidenced by Western blotting, immunofluorescence assay, quantitative PCR, and virus titration assay. Considering the current prevalence of NADC30-like and NADC34-like PRRSV2 of lineage 1 in China, the CD163-expressing macrophages are very useful for PRRSV research and disease management.

Keywords: CD163; MARC-145 cells; cell tropism; porcine reproductive and respiratory syndrome virus; stable macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Chlorocebus aethiops
  • Macrophages
  • Porcine Reproductive and Respiratory Syndrome*
  • Porcine respiratory and reproductive syndrome virus* / genetics
  • Porcine respiratory and reproductive syndrome virus* / metabolism
  • Receptors, Cell Surface
  • Receptors, Scavenger
  • Swine

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • Receptors, Cell Surface
  • Receptors, Scavenger

Grants and funding

The work was partly supported by the National Natural Science Foundation of China (31872450; 31802172) and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). N.C is supported by Natural Science Foundation for Excellent Young Scholars of Jiangsu Province (BK202111603) and High Talent Supporting Program of Yangzhou University. Y.X. is supported by Research Innovation Project of Jiangsu Province Graduate Students.