Serum antibodies to pneumococcal C polysaccharide in children: response to acute pneumococcal otitis media or to vaccination

Pediatr Infect Dis J. 1987 Jun;6(6):519-26. doi: 10.1097/00006454-198706000-00006.


Immunoglobulin class-specific antibody responses to the pneumococcal C polysaccharide (CPS) and to the capsular polysaccharides of types 3, 6A, 18C and 19F were measured by enzyme-linked immunosorbent assay in the sera of children ages 6 months to 7 years. Twenty of these children had acute otitis media caused by pneumococci of type/group 3, 6, 18 or 19, whereas 20 received an injection of 14-valent pneumococcal capsular polysaccharide vaccine. Many of the children in both groups had large concentrations of IgG and/or IgM class anti-CPS antibodies in their first serum sample. Both the infection and the vaccine elicited anti-CPS responses in all three immunoglobulin classes, most notably IgA. The pneumococcal capsular polysaccharides used as antigens in the enzyme-linked immunosorbent assay were found to contain CPS in amounts ranging from less than 1 to 30%. As a result the enzyme-linked immunosorbent assay detected both anti-type-specific and anti-CPS antibodies. After elimination of the anti-CPS, type-specific pneumococcal antibodies were found only occasionally and in low concentrations in serum samples obtained in the acute phase of otitis or before vaccination. The infection induced homologous type-specific pneumococcal antibodies to varying degrees depending on the type: regularly to type 3; and fairly regularly to type 18C polysaccharide, but seldom to type 6 or 19. The pneumococcal vaccine induced type-specific antibodies to all four types measured, but the response to type 6A was poor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antibodies, Bacterial / biosynthesis*
  • Bacterial Vaccines / immunology*
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoglobulin A / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / biosynthesis
  • Immunoglobulins / biosynthesis
  • Infant
  • Otitis Media / immunology*
  • Pneumococcal Infections / immunology*
  • Pneumococcal Vaccines
  • Polysaccharides, Bacterial / immunology*
  • Streptococcus pneumoniae / immunology*


  • Antibodies, Bacterial
  • Bacterial Vaccines
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulins
  • Pneumococcal Vaccines
  • Polysaccharides, Bacterial
  • polysaccharide C-substance (Streptococcus)