Bioinformatics of the Endocannabinoid System: Study of DNA Methylation at Rat Cnr1 Gene Promoter

Annalaura Sabatucci; Claudio D'Addario

doi:10.1007/978-1-0716-2728-0_30

Bioinformatics of the Endocannabinoid System: Study of DNA Methylation at Rat Cnr1 Gene Promoter

Methods Mol Biol. 2023:2576:361-371. doi: 10.1007/978-1-0716-2728-0_30.

Authors

Annalaura Sabatucci¹, Claudio D'Addario²

Affiliations

¹ Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. alsabatucci@unite.it.
² Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.

PMID: 36152202
DOI: 10.1007/978-1-0716-2728-0_30

Abstract

In this chapter, we will describe the bioinformatic tools that allow verifying the presence of CpG islands in a gene promoter region. We will also describe the tools needed to identify consensus motifs for specific transcription factors, focusing on the study of rat type-1 cannabinoid receptor gene (R_Cnr1) as a case study.

Keywords: CG methylation; Consensus motifs; CpG islands; Genome browsers; In silico methods; Rat cannabinoid receptor 1; Transcription factors.

MeSH terms

Animals
Computational Biology
CpG Islands
DNA Methylation*
Endocannabinoids* / genetics
Promoter Regions, Genetic
Rats
Receptor, Cannabinoid, CB1 / genetics
Receptors, Cannabinoid
Transcription Factors / genetics

Substances

Cnr1 protein, rat
Endocannabinoids
Receptor, Cannabinoid, CB1
Receptors, Cannabinoid
Transcription Factors