Membrane-Sensing Peptides for Extracellular Vesicle Analysis

Methods Mol Biol. 2023:2578:249-257. doi: 10.1007/978-1-0716-2732-7_18.

Abstract

Analytical platforms for small extracellular vesicle (sEV) high-throughput analysis are highly desirable. These bionanoparticles present fairly distinctive lipid membrane features including high curvature, lipid-packing defects, and a relative abundance in lipids. sEV membrane could be considered as a "universal" marker, complementary or alternative to traditional surface-associated proteins. Here, we describe the use of membrane-sensing peptides as a new, highly efficient ligand to directly integrate sEV capturing and analysis on a microarray platform.

Keywords: Extracellular vesicles; Membrane binding; Membrane curvature; Microarrays; Peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Extracellular Vesicles* / metabolism
  • Ligands
  • Lipids
  • Membrane Proteins / metabolism
  • Membranes / metabolism
  • Peptides* / metabolism

Substances

  • Ligands
  • Lipids
  • Membrane Proteins
  • Peptides