Analytical platforms for small extracellular vesicle (sEV) high-throughput analysis are highly desirable. These bionanoparticles present fairly distinctive lipid membrane features including high curvature, lipid-packing defects, and a relative abundance in lipids. sEV membrane could be considered as a "universal" marker, complementary or alternative to traditional surface-associated proteins. Here, we describe the use of membrane-sensing peptides as a new, highly efficient ligand to directly integrate sEV capturing and analysis on a microarray platform.
Keywords: Extracellular vesicles; Membrane binding; Membrane curvature; Microarrays; Peptides.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.