Hydrogen cyanide and cyanogen chloride formation by the myeloperoxidase-H2O2-Cl- system

Biochim Biophys Acta. 1979 Apr 12;567(2):309-14. doi: 10.1016/0005-2744(79)90116-5.


The chlorination of glycine by the myeloperoxidase-H2O2-Cl- system at acidic pH values yielded N-monochloroglycine and a mixture of HCN and ClCN. HCN was formed as a product of N-dichloroglycine decomposition and cyanogen chloride formation resulted from simultaneous chlorination of HCN by N-chloroglycine or directly by the myeloperoxidase-H2O2-Cl- system. HCN was readily chlorinated by the myeloperoxidase-H2O2Cl- system yielding cyanogen chloride. This dissociation constants of the myeloperoxidase-CN- complex were estimated as 2.5.10(-6)--1.15.10(-5) M within the pH range of 6.2 to 3.4, respectively. Chloride competed with cyanide for binding at the active site of myeloperoxidase. The lower the pH the more pronounced was the competitive effect of chloride. This accounted for chlorination by myeloperoxidase in the presence of CN-.

MeSH terms

  • Binding Sites
  • Chlorides / metabolism
  • Cyanides / metabolism*
  • Glycine / metabolism
  • Granulocytes / enzymology
  • Hydrogen Peroxide / metabolism
  • Hydrogen-Ion Concentration
  • Kinetics
  • Peroxidase / metabolism*
  • Peroxidases / metabolism*


  • Chlorides
  • Cyanides
  • Hydrogen Peroxide
  • Peroxidases
  • Peroxidase
  • Glycine