RNA-seq analysis revealing the immune response of Neocaridina denticulata sinensis gill to Vibrio parahaemolyticus infection

Fish Shellfish Immunol. 2022 Nov;130:409-417. doi: 10.1016/j.fsi.2022.09.049. Epub 2022 Sep 23.

Abstract

Vibrio parahaemolyticus causes serious economic losses to the shrimp farming industry. There is still a lack of adequate understanding of the changes in the overall response of N. denticulata sinensis caused by V. parahaemolyticus, particularly with respect to gill tissue, which is severely damaged by the pathogen. In this study, a total of 1358 differentially expressed genes were identified between the PBS control and Vibrio stimulation groups using transcriptome sequencing techniques. After further screening and analysis, many immune-related genes were obtained, involving lysosome pathway, metabolic process, chitin-binding protein, and serine protease family members. In addition, we randomly selected six DEGs in the lysosome pathway for qRT-PCR verification, and the results showed that their expression patterns were consistent with the RNA-seq. The results demonstrate the molecular regulation of the gill tissue response to V. parahaemolyticus infection in N. denticulata sinensis, contributing to the understand of the complex and efficient innate immune system and defense mechanisms in crustaceans.

Keywords: Gill; Immune response; Neocaridina denticulata sinensis; RNA-Seq analysis; Vibrio parahaemolyticus.

MeSH terms

  • Animals
  • Chitin
  • Decapoda* / genetics
  • Gene Expression Profiling / veterinary
  • Gills
  • Immunity, Innate / genetics
  • RNA-Seq
  • Serine Proteases
  • Vibrio Infections* / genetics
  • Vibrio Infections* / veterinary
  • Vibrio parahaemolyticus* / physiology

Substances

  • Chitin
  • Serine Proteases