The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties

Biochim Biophys Acta. 1979 Apr 12;567(2):315-24. doi: 10.1016/0005-2744(79)90117-7.

Abstract

The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors.

MeSH terms

  • Alcaligenes / enzymology*
  • Cell Membrane / enzymology*
  • Deoxycholic Acid
  • Hydrogen
  • Hydrogen-Ion Concentration
  • Iron-Sulfur Proteins
  • Methylene Blue / metabolism
  • Molecular Weight
  • Oxidoreductases / isolation & purification
  • Oxidoreductases / metabolism*
  • Phosphatidylcholines / pharmacology
  • Polyethylene Glycols
  • Solubility
  • Substrate Specificity

Substances

  • Iron-Sulfur Proteins
  • Phosphatidylcholines
  • Deoxycholic Acid
  • Polyethylene Glycols
  • Hydrogen
  • Oxidoreductases
  • Methylene Blue