miR-515-5p suppresses trophoblast cell invasion and proliferation through XIAP regulation in preeclampsia

Mol Cell Endocrinol. 2023 Jan 1:559:111779. doi: 10.1016/j.mce.2022.111779. Epub 2022 Sep 22.

Abstract

MicroRNAs (miRNAs) are non-coding small RNA molecules that can be secreted into the circulation and which exist in remarkably stable forms. Circulating miRNAs regulate numerous biological process and are aberrantly expressed in pathological conditions. Differentially expressed circulating miRNAs have received attention as potential biomarkers for many diseases. In this study, we revealed that miR-515-5p was significantly upregulated in maternal serum from preeclampsia patients in comparison to normal pregnant women. Bioinformatics prediction and a dual-luciferase reporter gene assay revealed that miR-515-5p directly targets the X-linked inhibitor of apoptosis protein (XIAP) 3'-untranslated region. In addition, the overexpression of miR-515-5p inhibited the proliferation and invasion of HTR-8/SVneo trophoblast cells. The decreased XIAP expression and reduced epithelial-mesenchymal transition (EMT) were observed in the preeclamptic placenta. Collectively, miR-515-5p may play critical roles in the pathogenesis of preeclampsia through suppression of XIAP, and serum miR-515-5p may act as a potential biomarker for preeclampsia.

Keywords: Invasion; Preeclampsia; Proliferation; Trophoblast; XIAP; miR-515-5p.

MeSH terms

  • Biomarkers / metabolism
  • Cell Line
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Female
  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Pre-Eclampsia* / metabolism
  • Pregnancy
  • Trophoblasts / metabolism
  • X-Linked Inhibitor of Apoptosis Protein / genetics
  • X-Linked Inhibitor of Apoptosis Protein / metabolism

Substances

  • X-Linked Inhibitor of Apoptosis Protein
  • MicroRNAs
  • Biomarkers
  • XIAP protein, human
  • MIRN515 microRNA, human