Rapid quantification of cannabidiol from oils by direct analysis in real time mass spectrometry

Anal Methods. 2022 Oct 13;14(39):3875-3880. doi: 10.1039/d2ay01229d.


This work is the first to describe the use of Direct Analysis in Real Time Mass Spectrometry (DART-MS) for the rapid quantification of cannabidiol (CBD) in CBD oils. For this study, self-prepared samples spiked with CBD in hemp seed oil as well as commercial CBD oils from the Austrian market with different CBD contents were analyzed. CBD concentrations were between 5 and 30% (m/m) for the spiked samples as well as between 5 and 15% (m/m) for the real samples. The performance of quantification by means of DART-MS was assessed against a validated liquid chromatography-mass spectrometry (LC-MS) method. The correlation of the quantification results of both methods was high with a correlation factor greater than 0.98 and a maximum bias of 9.8%. Furthermore, the relative standard deviation values of the DART-MS measurments were below the tolerable limit of 12%. These results demonstrate that quantification of CBD by DART-MS is reliable and hence suitable as a rapid and cost-effective alternative method for quality control of CBD content in CBD oils.

MeSH terms

  • Cannabidiol* / analysis
  • Cannabidiol* / chemistry
  • Cannabis* / chemistry
  • Mass Spectrometry
  • Plant Extracts
  • Plant Oils


  • Plant Extracts
  • Plant Oils
  • Cannabidiol
  • hempseed oil