SAG-dihydrochloride enhanced the expression of germ cell markers in the human bone marrow- mesenchymal stem cells (BM-MSCs) through the activation of GLI-independent hedgehog signaling pathway

Gene. 2023 Jan 15;849:146902. doi: 10.1016/j.gene.2022.146902. Epub 2022 Sep 19.

Abstract

Different studies indicated that the enhancing the expression of germ cell markers improved the efficiency of stem cells in the generation of germ line cells. The aim of the present study was to investigate the effect of SAG-dihydrochloride on the expression of germ cell markers in the human bone marrow-mesenchymal stem cells (BM-MSCs). For this purpose, the human BM-MSCs were cultured in the medium containing different concentrations of SAG-dihydrochloride (10, 20 and 30 µM). After RNA extraction and cDNA synthesis, the expression level of PTCH1, GLI1, PLZF, DDX4 and STRA8 genes were determined by using SYBR Green Real time PCR. The analysis of the results obtained from PTCH1 and GLI1 expression indicated that SAG-dihydrochloride had the ability to enhance the expression of germ cell markers in a Gli-independent manner. Furthermore, the significant increased expression of STRA8 was observed in the BM-MSCs treated by 10 µM SAG-dihydrochloride for 4 and 6 days (p < 0.05). There was also the up-regulation of DDX4 in the BM-MSCs following treatment with 20 µM SAG-dihydrochloride for 4 and 6 days. The obtained results suggested that treatment with SAG-dihydrochloride increased the expression of germ cell markers in the human BM-MSCs through the activation of non-canonical sonic hedgehog signaling pathway.

Keywords: Germ cell markers; Infertility; Mesenchymal stem cells; SAG-dihydrochloride; Sonic hedgehog signaling pathway.

MeSH terms

  • Bone Marrow / metabolism
  • Bone Marrow Cells* / metabolism
  • Cell Differentiation / genetics
  • DNA, Complementary
  • Germ Cells / metabolism
  • Hedgehog Proteins / genetics
  • Hedgehog Proteins / metabolism
  • Humans
  • Mesenchymal Stem Cells* / metabolism
  • RNA
  • Zinc Finger Protein GLI1 / genetics

Substances

  • Hedgehog Proteins
  • DNA, Complementary
  • Zinc Finger Protein GLI1
  • RNA