Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics

STAR Protoc. 2022 Dec 16;3(4):101720. doi: 10.1016/j.xpro.2022.101720. Epub 2022 Sep 27.


Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021).

Keywords: Cancer; Cell biology; Cell isolation; Cell-based assays; Clinical protocol; Health sciences; High throughput screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Epithelial Cells
  • Humans
  • Lung Neoplasms*