Sphingomonas baiyangensis sp. nov., isolated from water in Baiyang Lake

Int J Syst Evol Microbiol. 2022 Sep;72(9). doi: 10.1099/ijsem.0.005326.

Abstract

A novel Gram-stain-negative, short-rod-shaped, orange-pigmented bacterial strain, designated L-1-4 w-11T, was isolated from Baiyang Lake in China. The strain grew at 15-35 °C (optimum 30 °C) and pH 7-8 (optimum pH 7) in TSA medium. The predominant polar lipids of strain L-1-4 w-11T were sphingoglycolipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and three unidentified lipids; the major cellular fatty acids were C17 : 1ω6c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c); and the major respiratory quinone was ubiquinone 10 (Q-10). Strain L-1-4 w-11T showed the highest 16S rRNA gene sequence similarity to Sphingomonas japonica JCM 15438T (98.3 %) and S. spermidinifaciens GDMCC 1.657T (98.0 %). The draft genome size of strain L-1-4 w-11T was 3.3 Mbp, and the G+C content was 67.8 mol%. Digital DNA-DNA hydridization and average nucleotide identity values between the genome sequences of strain L-1-4 w-11T and S. spermidinifaciens GDMCC 1.657T (76.9 and 21.0 %), S. japonica JCM 15438T (76.0 and 19.9 %) and S. paucimobilis CGMCC 1.12825T (72.8 and 19.6 %) were far below the thresholds for prokaryotic conspecific assignment. With the evidence from the phylogenetic, chemotaxonomic and genotypic analyses, we propose that strain L-1-4 w-11T represents a novel Sphingomonas species with the name S. baiyangensis sp. nov. The type strain is L-1-4 w-11T (=CGMCC 1.13572T=JCM 33962T).

Keywords: Baiyang Lake; Sphingomonas baiyangensis sp. nov; freshwater.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Cardiolipins
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Glycolipids / chemistry
  • Glycosphingolipids
  • Lakes / microbiology
  • Nucleotides
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids / chemistry
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology
  • Sphingomonas*
  • Water

Substances

  • Cardiolipins
  • DNA, Bacterial
  • Fatty Acids
  • Glycolipids
  • Glycosphingolipids
  • Nucleotides
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Water