Regulatory T-Cell Phenotyping Using CyTOF

Hugo Barcenilla; Mikael Pihl; Florence Sjögren; Louise Magnusson; Rosaura Casas

doi:10.1007/978-1-0716-2647-4_15

Regulatory T-Cell Phenotyping Using CyTOF

Methods Mol Biol. 2023:2559:231-242. doi: 10.1007/978-1-0716-2647-4_15.

Authors

Hugo Barcenilla¹, Mikael Pihl², Florence Sjögren², Louise Magnusson³, Rosaura Casas³

Affiliations

¹ Department of Biomedical and Clinical Sciences, Division of Children's and Women's Health, Faculty of Health Sciences, Linköping University, Linköping, Sweden. hugo.barcenilla@liu.se.
² Core Facility, Flow cytometry Unit, Linköping University, Linköping, Sweden.
³ Department of Biomedical and Clinical Sciences, Division of Children's and Women's Health, Faculty of Health Sciences, Linköping University, Linköping, Sweden.

PMID: 36180636
DOI: 10.1007/978-1-0716-2647-4_15

Abstract

Regulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3.

Keywords: CyTOF; Foxp3; Mass cytometry; Regulatory T cells; Single-cell analysis; Treg; immunophenotyping; phenotyping.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flow Cytometry / methods
Forkhead Transcription Factors / genetics
Immunophenotyping
Metals, Heavy*
T-Lymphocyte Subsets
T-Lymphocytes, Regulatory*

Substances

Forkhead Transcription Factors
Metals, Heavy