Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats

Methods Mol Biol. 2022:2550:53-62. doi: 10.1007/978-1-0716-2593-4_8.


The sympathetic nervous system has been implicated in various physiological and pathological processes, including regulation of homeostatic functions, maintenance of the circadian rhythms, and neuronal disruption and recovery after injury. Of special interest is focus on the role of the superior cervical ganglion (SCG) in regulating the daily changes in pineal function. Removal of the superior cervical ganglion (SCGx) and decentralization have served as valuable microsurgical models to investigate the effects of surgical denervation on this gland or organ. In this chapter, we offer information about methodologies for performing SCGx along with decentralization and denervation procedures, including details about recommended equipment as well as tips that can improve these techniques.

Keywords: Decentralization; Denervation; Rat; Superior cervical ganglia; Superior cervical ganglionectomy; Surgical procedure.

MeSH terms

  • Animals
  • Circadian Rhythm / physiology
  • Ganglia, Sympathetic
  • Ganglionectomy* / methods
  • Neurons
  • Politics
  • Rats
  • Superior Cervical Ganglion*