Analysis of the Human Pineal Proteome by Mass Spectrometry

Methods Mol Biol. 2022;2550:123-132. doi: 10.1007/978-1-0716-2593-4_16.


The human pineal gland regulates the day-night dynamics of multiple physiological processes, especially through the secretion of melatonin. Recently, using mass spectrometry-based proteomics and dedicated analysis tools, we have identified regulated proteins and signaling pathways that differ between day and night and/or between control and autistic pineal glands. This large-scale proteomic approach is the method of choice to study proteins in a biological system globally. This chapter proposes a protocol for large-scale analysis of the pineal gland proteome.

Keywords: Autism; Human; Mass spectrometry; Pineal gland; Protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circadian Rhythm / physiology
  • Humans
  • Mass Spectrometry
  • Melatonin* / metabolism
  • Pineal Gland* / metabolism
  • Proteome / metabolism
  • Proteomics / methods


  • Proteome
  • Melatonin