Conjugates of Desmycosin with Fragments of Antimicrobial Peptide Oncocin: Synthesis, Antibacterial Activity, Interaction with Ribosome

Biochemistry (Mosc). 2022 Sep;87(9):871-889. doi: 10.1134/S0006297922090024.


Design and synthesis of conjugates consisting of the macrolide antibiotic desmycosin and fragments of the antibacterial peptide oncocin were performed in attempt to develop new antimicrobial compounds. New compounds were shown to bind to the E. coli 70S ribosomes, to inhibit bacterial protein synthesis in vitro, as well as to suppress bacterial growth. The conjugates of N-terminal hexa- and tripeptide fragments of oncocin and 3,2',4''-triacetyldesmycosin were found to be active against some strains of macrolide-resistant bacteria. By simulating molecular dynamics of the complexes of these compounds with the wild-type bacterial ribosomes and with ribosomes, containing A2059G 23S RNA mutation, the specific structural features of their interactions were revealed.

Keywords: antimicrobial peptides; macrolides; molecular dynamics simulations; nascent peptide exit tunnel; peptide derivatives; ribosome.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Antimicrobial Cationic Peptides / pharmacology
  • Antimicrobial Peptides*
  • Bacterial Proteins / metabolism
  • Escherichia coli* / metabolism
  • Macrolides / analysis
  • Macrolides / metabolism
  • Protein Synthesis Inhibitors / chemistry
  • RNA / metabolism
  • Ribosomes / chemistry
  • Tylosin / analogs & derivatives


  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Antimicrobial Peptides
  • Bacterial Proteins
  • Macrolides
  • Protein Synthesis Inhibitors
  • oncocin
  • tylosin B
  • RNA
  • Tylosin