Deficiency of a peroxisomal NADP-isocitrate dehydrogenase leads to dwarf plant and defect seed in upland cotton

Yuefen Cao; Wanru Wang; Jinhong Chen; Shuijin Zhu; Tianlun Zhao

doi:10.3389/fpls.2022.1000883

Deficiency of a peroxisomal NADP-isocitrate dehydrogenase leads to dwarf plant and defect seed in upland cotton

Front Plant Sci. 2022 Sep 14:13:1000883. doi: 10.3389/fpls.2022.1000883. eCollection 2022.

Authors

Yuefen Cao^{1

2}, Wanru Wang^{1

2}, Jinhong Chen^{1

2}, Shuijin Zhu^{1

2

3}, Tianlun Zhao^{1

2

3}

Affiliations

¹ College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
² Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, China.
³ Hainan Institute, Zhejiang University, Sanya, China.

Abstract

The NADP-isocitrate dehydrogenase-encoded gene GH_D13G1452 with a C-terminus tripeptide Proline-Lysine-Leucine was localized in the peroxisome. It was highly expressed in stems and ovules of 15 days post-anthesis and responded to multiple external stimuli in upland cotton. An upland cotton mutant (Ghpericdh) was identified by flanking sequence amplification and genome variation detection that exogenous sequence was inserted in the middle of the 12th intron of GH_D13G1452, resulting in the deficiency of gene expression. The Ghpericdh mutant displayed a dwarf plant phenotype when grown under field or greenhouse conditions, and GH_D13G1452 functioned as an incomplete dominance on plant height. The germination rate of mutant seed from greenhouse-grown plants was dramatically lower than that from field-grown plants, which indicated that GhperICDH plays a critical role in seed maturation and germination. Therefore, GH_D13G1452 is indispensable in the development of stems and seeds and functions in the adaptability of cotton to the environment. The Ghpericdh mutant provides insight into the function of peroxisomal ICDH and may contribute to the genetic improvement in cotton.

Keywords: Gossypium; perICDH; plant height; seed development; subcellular localization.