Culturomic, metagenomic, and transcriptomic-based characterization of commensal lactic acid bacteria isolated from domestic dogs using Caenorhabditis elegans as a model for aging

J Anim Sci. 2022 Oct 4;skac323. doi: 10.1093/jas/skac323. Online ahead of print.

Abstract

In tandem with the fast expansion of the pet-economy industry, the present ageing research has been noticing the function of probiotics in extending the healthy lifetime of domestic animals. In this study, we aimed to understand the bacterial compositions of canine feces and isolating lactic acid bacteria as commensal lactic acid bacteria (LAB) as novel potential probiotics for the use of anti-aging using Caenorhabditis elegans surrogate animal model. Under an anaerobic culturomic and metagenomic analysis, a total of 305 commensal LAB were isolated from diverse domestic dogs, and four strains, Lactobacillus amylolyticus, L. salivarius, Enterococcus hirae and E. faecium, made prominence as commensal LAB by enhancing C. elegans life span and restored neuronal degeneration induced by aging by up-regulating skn-1, ser-7 and odr-3, 7, 10. Importantly, whole transcriptome results and integrative network analysis revealed extensive mRNA encoding protein domains and functional pathways of naturally aging C. elegans was examined and we built the gene informatics basis. Taken together, our finding proposed that a specific gene network corresponding to the pathways differentially expressed during the aging and selected commensal LAB as potential probiotic strains could be provided beneficial effects in the aging of domestic animals by modulating dynamics of gut microbiota.

Keywords: Commensal LAB; anti-aging; domestic animal; gut microbiome; multiomics.