Kinetics of Benzo(a)pyrene biodegradation and bacterial growth in sandy soil by Sphingobacterium spiritovorum

Heliyon. 2022 Oct 1;8(10):e10799. doi: 10.1016/j.heliyon.2022.e10799. eCollection 2022 Oct.


Biodegradation is the economically viable solution to restore land contaminated by hazardous pollutants such as benzo(a)pyrene (BaP). The present study focuses on the biodegradation of benzo(a)pyrene by Sphingobacterium spiritovorum in contaminated soil. The biodegradation kinetics and bacterial growth were evaluated while the biokinetic model that described the benzo(a)pyrene biodegradation was established. The Monod, Haldane, Powell and Edward models were used to model the bacterial growth in benzo(a)pyrene contaminated soil. Excel template was developed with Fourth order Runga-Kutta numerical algorithm to find the biokinetic parameters of the complex non-linear regression model. An Excel Solver function was used to obtain reasonable best-fit values of kinetic parameters. The Haldane and Edward models are well fit to describe the growth trend and model the kinetics of benzo(a)pyrene biodegradation. Enzyme substrate inhibition is the critical factor that affects the benzo(a)pyrene degradation by S. spiritovorum, which the model defines physically. The results demonstrated that removing benzo(a)pyrene showed positive interaction between substrate inhibition, the concentration of benzo(a)pyrene and sorption of the contaminants on soil particles.

Keywords: Benzo(a)pyrene; Biodegradation; Monod; Polycyclic aromatic hydrocarbon (PAH); Sphingobacterium.