Although numerous studies have evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using cycle threshold (Ct) values as a surrogate of viral ribonucleic acid (RNA) load, few studies have used standardized, quantitative methods. We validated a quantitative SARS-CoV-2 digital polymerase chain reaction assay normalized to World Health Organization International Units and correlated viral RNA load with symptoms and disease severity.
Keywords: COVID-19; SARS-CoV-2; digital PCR; international units; viral load.
© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.