Fluorescence microscopy assays enable the investigation of endogenous biomolecular condensates directly in their cellular context. With appropriate experimental designs, these assays yield quantitative information on condensate material properties and inform on biophysical mechanisms of condensate formation. Single-molecule super-resolution and tracking experiments grant access to the smallest condensates and early condensation stages not resolved by conventional imaging approaches. Here, we discuss considerations for using single-molecule assays to extract quantitative information about biomolecular condensates directly in their cellular context.
Keywords: Liquid-liquid phase separation; Multiphase assembly; Single-molecule tracking; Super-resolution microscopy.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.