Sub-compartmentation of the 'cytosolic' glucose 6-phosphate pool in cultured rat hepatocytes

FEBS Lett. 1987 Sep 14;221(2):375-80. doi: 10.1016/0014-5793(87)80959-6.


[14C]Glucose release either from endogenous 14C-prelabelled glycogen or from added 14C-labelled glucose 6-phosphate was measured in filipin-treated, permeabilized hepatocytes in 48 h culture. [14C]Glucose output from prelabelled glycogen was not altered by the addition of 5 mM glucose 6-phosphate to the incubation medium. Conversely, [14C]glucose release from 5 mM labelled glucose 6-phosphate was not influenced by different glycogen concentrations in the cells. Moreover, in the permeabilized cells the anion transport inhibitor DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) inhibited only the liberation of [14C]glucose from labelled glucose 6-phosphate but not from glycogen. It is therefore concluded that there exist at least 2 separate, mutually non-accessible glucose 6-phosphate pools in cultured rat hepatocytes, one linked to glycogenolysis and the other to gluconeogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
  • Animals
  • Calcium / metabolism
  • Cell Membrane Permeability
  • Cells, Cultured
  • Cytosol / analysis
  • Gluconeogenesis
  • Glucose / metabolism
  • Glucose-6-Phosphate
  • Glucosephosphates / analysis*
  • Glycogen / metabolism
  • Liver / analysis*
  • Male
  • Rats
  • Rats, Inbred Strains


  • Glucosephosphates
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Glucose-6-Phosphate
  • Glycogen
  • Glucose
  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • Calcium