A dual-site multifunctional fluorescent probe for selective detection of endogenous H2O2 and SO2 derivatives based on ICT process and its bioimaging application

Abstract

In this paper, we reported a coumarin-based fluorescent probe for selective detection of H₂O₂/SO₂ derivatives via ICT process. To the best of our knowledge, it was few reported with the same probe to enable visual detection of H₂O₂/SO₂ derivatives in vivo and in vitro. H₂O₂ and SO₃^2- were selectively sensed over other analytes, and the probe displayed 20-fold and 220-fold relative fluorescence intensity respectively, as well as the good linear relationship and the excellent detection limits of 2.7 * 10³ nM and 19.3 nM. Furthermore, the probe was successfully used for fluorescence imaging of the HeLa cells and the mice to monitor exogenous and endogenous H₂O₂ and SO₃^2-, suggesting its potential biomedical application for investigation and detection the intermediate indicator of oxidative stress in vitro and in vivo.

Keywords: Dual-site; Fluorescent probe; Hydrogen peroxide; Imaging; SO(2) derivatives.