Detecting aquatic pathogens with field-compatible dried qPCR assays

J Microbiol Methods. 2022 Nov:202:106594. doi: 10.1016/j.mimet.2022.106594. Epub 2022 Oct 12.

Abstract

Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

Keywords: Air-dried assay; Aquatic pathogens; Environmental diagnostics; Lyophilization; eDNA; qPCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphanomyces* / genetics
  • Astacoidea*
  • Real-Time Polymerase Chain Reaction