Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.
Keywords: Air-dried assay; Aquatic pathogens; Environmental diagnostics; Lyophilization; eDNA; qPCR.
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.