Structures of novel sialylated O-linked oligosaccharides isolated from human erythrocyte glycophorins

J Biol Chem. 1987 Sep 5;262(25):11952-7.


The O-linked oligosaccharides attached to human erythrocyte glycophorins were extensively characterized. In addition to the previously described disialylated tetrasaccharide, NeuNAc alpha 2----3Gal beta 1----3 (Neu-NAc alpha 2----6)GalNAcOH and monosialylated trisaccharide, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, novel trisialylated oligosaccharides were isolated. Methylation analysis, fast atom bombardment-mass spectrometry, and enzymatic degradation were used to elucidate the following novel structures: formula; see text: These results suggest that O-linked oligosaccharides with a disialosyl group, NeuNAc alpha 2----8NeuNAc alpha 2----, may be present in various tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbohydrate Conformation
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Glycophorins / analysis*
  • Humans
  • Mass Spectrometry
  • Methylation
  • Oligosaccharides / analysis*
  • Sialic Acids / analysis
  • Sialoglycoproteins / analysis*


  • Glycophorins
  • Oligosaccharides
  • Sialic Acids
  • Sialoglycoproteins