Production of monoclonal antibodies against RNA polymerase I from nonimmunized autoimmune MRL/lpr mice and their use in rDNA transcription analysis

J Biol Chem. 1987 Sep 15;262(26):12759-63.


A hybridoma secreting monoclonal antibodies against RNA polymerase I was produced by the fusion of myeloma cells with spleen cells from a nonimmunized MRL/lpr mouse which is known to produce autoantibodies to RNA polymerase I. The antibodies (McAb-2D11) belong to the IgG2b subclass, reacted specifically with the second largest (120 kDa) subunit of RNA polymerase I, and inhibited accurate transcription of cloned rat rDNA in a fractionated cell extract following immunoprecipitation of RNA polymerase I. McAb-2D11 did not inhibit RNA polymerase II-mediated transcription of the mouse metallothionein-I gene. Immunocytochemical procedures with biotinylated second antibody demonstrated specific immunolocalization of RNA polymerase I in the nucleus. These studies have (a) presented direct evidence that autoantibodies to functional RNA polymerase I are produced in a murine model of systemic lupus erythematosus, (b) demonstrated specificity of the monoclonal antibody for RNA polymerase I, and (c) provided a useful tool for the purification of RNA polymerase I and/or transcription factor(s) associated with RNA polymerase I.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Autoimmune Diseases / immunology*
  • DNA, Ribosomal / analysis*
  • Immunoglobulin G / immunology
  • Liver Neoplasms, Experimental / enzymology
  • Lupus Erythematosus, Systemic / immunology*
  • Mice
  • Mice, Inbred Strains / immunology*
  • Mice, Mutant Strains / immunology*
  • Neoplasm Proteins / immunology
  • RNA Polymerase I / antagonists & inhibitors
  • RNA Polymerase I / immunology*
  • RNA, Ribosomal / biosynthesis
  • Transcription, Genetic


  • Antibodies, Monoclonal
  • DNA, Ribosomal
  • Immunoglobulin G
  • Neoplasm Proteins
  • RNA, Ribosomal
  • RNA Polymerase I