Simultaneous determination of zopiclone and its two major metabolites (N-oxide and N-desmethyl) in human biological fluids by reversed-phase high-performance liquid chromatography

J Chromatogr. 1987 Jun 5;417(1):151-8. doi: 10.1016/0378-4347(87)80101-9.


A high-performance liquid chromatographic method has been developed for the simultaneous determination of zopiclone and its main metabolites (N-oxide and N-desmethyl derivatives) in biological fluids. After selective extraction (dichloromethane-2-propanol) these compounds are chromatographed on a column packed with Spherisorb ODS-2 (5 micron) using monobasic sodium phosphate-methanol (45:55, v/v). The eluted compounds are measured by fluorescence detection. The limit of detection of the method is 5 ng/ml for zopiclone in plasma and urine and 10 ng/ml for its two main metabolites (coefficient of variation less than 10%). This method has been successfully applied to pharmacokinetic studies of zopiclone and its two main metabolites in healthy subjects and patients with chronic renal failure.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Azabicyclo Compounds
  • Chromatography, High Pressure Liquid
  • Humans
  • Hypnotics and Sedatives / analysis*
  • Hypnotics and Sedatives / blood
  • Hypnotics and Sedatives / urine
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / urine
  • Kinetics
  • Middle Aged
  • Piperazines / analysis*
  • Piperazines / blood
  • Piperazines / urine
  • Spectrometry, Fluorescence


  • Azabicyclo Compounds
  • Hypnotics and Sedatives
  • Piperazines
  • zopiclone
  • zopiclone N-oxide
  • N-desmethylzopiclone