Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis

Debbie van der Burg; Hermann Wätzig; Cari E Sänger-van de Griend

doi:10.1155/2022/2819855

Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis

J Anal Methods Chem. 2022 Oct 6:2022:2819855. doi: 10.1155/2022/2819855. eCollection 2022.

Authors

Debbie van der Burg^{1

2

3}, Hermann Wätzig³, Cari E Sänger-van de Griend¹

Affiliations

¹ Kantisto BV, Callenburglaan 22, 3742 MV, Baarn, Netherlands.
² KTH Royal Institute of Technology, Department of Chemistry, Division of Applied Physical Chemistry, Stockholm, Sweden.
³ Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.

Abstract

This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10-110 µM for undiluted samples, with 10 μM being the lower range limit of quantification QL, showed accuracy recoveries of 94-104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively.