Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges

Abstract

Systemic administration of histone deacetylase inhibitors (HDACi), like valproic acid (VPA), is often associated with rapid drug metabolization and untargeted tissue distribution. This requires high-dose application that can lead to unintended side effects. Hence, drug carrier systems such as nanoparticles (NPs) are developed to circumvent these disadvantages by enhancing serum half-life as well as organ specificity.This chapter gives a summary of the biological characterization of HDACi-coupled NPs in vitro, including investigation of cellular uptake, biocompatibility, as well as intracellular drug release and activity. Suitable methods, opportunities, and challenges will be discussed to provide general guidelines for the analysis of HDACi drug carrier systems with a special focus on recently developed cellulose-based VPA-coupled NPs.

Keywords: Biocompatibility; Cellular uptake; Cellulose; Drug delivery system; Hemocompatibility; Histone deacetylase inhibitor; Imaging; Nanoparticle; Shell-less hen’s egg model; Valproic acid.