Perturbation of Gene Regulation by Genome Editing

Nan Cher Yeo; George M Church

doi:10.1007/978-1-0716-2815-7_5

Perturbation of Gene Regulation by Genome Editing

Methods Mol Biol. 2023:2594:59-68. doi: 10.1007/978-1-0716-2815-7_5.

Authors

Nan Cher Yeo^{1

2}, George M Church^{3

4}

Affiliations

¹ Precision Medicine Institute, University of Alabama-Birmingham, Birmingham, AL, USA. nyeo@uab.edu.
² Department of Pharmacology and Toxicology, University of Alabama-Birmingham, Birmingham, AL, USA. nyeo@uab.edu.
³ Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA. gchurch@genetics.med.harvard.edu.
⁴ Department of Genetics, Harvard Medical School, Boston, MA, USA. gchurch@genetics.med.harvard.edu.

PMID: 36264488
DOI: 10.1007/978-1-0716-2815-7_5

Abstract

The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor. Here we describe a set of protocols for using the catalytically inactive dead Cas9 (dCas9)-based tools, including the bipartite super repressor consisting of the KRAB and MeCP2 domains, to achieve efficient and scalable gene silencing in mammalian cells.

Keywords: CRISPR-Cas transcriptional repressor; Single and multiplex gene silencing; dCas9-KRAB-MeCP2.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Animals
CRISPR-Cas Systems*
Endonucleases / genetics
Gene Editing*
Gene Expression Regulation
Mammals / genetics
RNA

Substances

Endonucleases
RNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding