A nuclear magnetic resonance study of the role of phosphoenol pyruvate carboxykinase (PEPCK) in the glucose metabolism of Dipetalonema viteae

Mol Biochem Parasitol. 1987 Jun;24(2):125-30. doi: 10.1016/0166-6851(87)90098-3.

Abstract

13C-NMR has been applied to the study of the metabolism of [1-13C]glucose by macrofilariae of Dipetalonema viteae under conditions of restricted glucose supply. In a medium buffered with 13C-labelled bicarbonate, succinate labelled in the carboxyl position is formed in good yield. Quinolinic acid, a known inhibitor of phosphoenol pyruvate carboxykinase (PEPCK) has been shown to suppress the formation of labelled succinate from [1-13C]glucose. Both sets of experiments support the formation of succinate through the PEPCK-mediated carboxylation of phosphoenol pyruvate, followed by the operation of a partial tricarboxylic acid cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dipetalonema / enzymology*
  • Female
  • Glucose / metabolism*
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Male
  • Phosphoenolpyruvate Carboxykinase (GTP) / metabolism*
  • Quinolinic Acids / pharmacology

Substances

  • Quinolinic Acids
  • Phosphoenolpyruvate Carboxykinase (GTP)
  • Glucose