Methods for Studying Appendicular Skeletal Biology in Axolotls

Camilo Riquelme-Guzmán; Tatiana Sandoval-Guzmán

doi:10.1007/978-1-0716-2659-7_9

Methods for Studying Appendicular Skeletal Biology in Axolotls

Methods Mol Biol. 2023:2562:155-163. doi: 10.1007/978-1-0716-2659-7_9.

Authors

Camilo Riquelme-Guzmán¹, Tatiana Sandoval-Guzmán²

Affiliations

¹ Medical Faculty: Department of Internal Medicine 3, Center for Healthy Aging, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
² Medical Faculty: Department of Internal Medicine 3, Center for Healthy Aging, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. tatiana.sandoval_guzman@tu-dresden.de.

PMID: 36272073
DOI: 10.1007/978-1-0716-2659-7_9

Abstract

The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.

Keywords: Calcification; Cartilage/bone; Live staining; Skeleton.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcian Blue
Ambystoma mexicanum*
Animals
Biology*
Staining and Labeling
X-Ray Microtomography

Substances

Alcian Blue
alizarin