Photo-repair effect of a bacterial Antarctic CPD-photolyase on UVC-induced DNA lesions in human keratinocytes

Environ Toxicol Pharmacol. 2022 Nov:96:104001. doi: 10.1016/j.etap.2022.104001. Epub 2022 Oct 20.

Abstract

Exposure to ultraviolet radiation from sunlight induces oxidative DNA lesions and bipyrimidine photoproducts that can lead to photo-aging and skin carcinogenesis. CPD-photolyases are flavoproteins that repair cyclobutane pyrimidine dimers using blue light as an energy source. In the present work, we evaluated the photo-repair effect of the recombinant CPD-photolyase PhrAHym from the Antarctic bacterium Hymenobacter sp. UV11 on DNA lesions in human keratinocytes induced by UVC light. By performing immunochemistry assays we observed that PhrAHym repairs in a highly efficient way the CPD-photoproducts and reduces the γH2AX formation. Since this enzyme is non-cytotoxic and repairs UVC-induced DNA lesions in human keratinocytes, we propose that PhrAHym could be used as a biotherapeutic agent against UV-induced skin cancer, photoaging, and related diseases.

Keywords: Cyclobutane pyrimidine dimers; DNA damage; Photo-repair; Photolyase; Ultraviolet light; γH2AX.

MeSH terms

  • Bacteria / enzymology
  • Bacteria / genetics
  • DNA Damage*
  • DNA Repair
  • Deoxyribodipyrimidine Photo-Lyase* / genetics
  • Deoxyribodipyrimidine Photo-Lyase* / metabolism
  • Humans
  • Keratinocytes* / metabolism
  • Keratinocytes* / radiation effects
  • Ultraviolet Rays / adverse effects

Substances

  • Deoxyribodipyrimidine Photo-Lyase