The human c-Kirsten ras gene is activated by a novel mutation in codon 13 in the breast carcinoma cell line MDA-MB231

Nucleic Acids Res. 1987 Aug 11;15(15):5963-71. doi: 10.1093/nar/15.15.5963.


We have detected amplified human Ki-ras sequences in tumorigenic NIH 3T3 cells transfected with genomic DNA from the human breast carcinoma cell line MDA-MB231. Hybridization of synthetic oligonucleotides specific for human Ki-ras sequences showed a mutation at codon 13. The polymerase chain reaction with Ki-ras specific amplimers revealed a guanosine to adenosine transition at the second position of codon 13, resulting in a substitution of glycine by aspartic acid. The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Cell Line
  • Codon*
  • Female
  • Gene Amplification
  • Humans
  • Mice
  • Mutation*
  • Proto-Oncogenes*
  • RNA, Messenger*
  • Transfection


  • Codon
  • RNA, Messenger