The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6

J Integr Plant Biol. 2023 Mar;65(3):854-875. doi: 10.1111/jipb.13399. Epub 2023 Feb 23.


NAC transcription factors (TFs) play critical roles in plant immunity by modulating the expression of downstream genes via binding to specific cis-elements in promoters. Here, we report the function and regulatory network of a pathogen- and defense phytohormone-inducible NAC TF gene, ONAC083, in rice (Oryza sativa) immunity. ONAC083 localizes to the nucleus and exhibits transcriptional activation activity that depends on its C-terminal region. Knockout of ONAC083 enhances rice immunity against Magnaporthe oryzae, strengthening pathogen-induced defense responses, and boosting chitin-induced pattern-triggered immunity (PTI), whereas ONAC083 overexpression has opposite effects. We identified ONAC083-binding sites in the promoters of 82 genes, and showed that ONAC083 specifically binds to a conserved element with the core sequence ACGCAA. ONAC083 activated the transcription of the genes OsRFPH2-6, OsTrx1, and OsPUP4 by directly binding to the ACGCAA element. OsRFPH2-6, encoding a RING-H2 protein with an N-terminal transmembrane region and a C-terminal typical RING domain, negatively regulated rice immunity against M. oryzae and chitin-triggered PTI. These data demonstrate that ONAC083 negatively contributes to rice immunity against M. oryzae by directly activating the transcription of OsRFPH2-6 through the ACGCAA element in its promoter. Overall, our study provides new insight into the molecular regulatory network of NAC TFs in rice immunity.

Keywords: ACGCAA element; Magnaporthe oryzae; ONAC083; OsRFPH2-6; immunity; rice.

MeSH terms

  • Chitin / metabolism
  • Disease Resistance
  • Gene Expression Regulation, Plant
  • Magnaporthe* / physiology
  • Oryza* / genetics
  • Plant Diseases / genetics
  • Plant Immunity / physiology
  • Plant Proteins / metabolism
  • Transcription Factors / metabolism


  • Transcription Factors
  • Chitin
  • Plant Proteins

Supplementary concepts

  • Pyricularia oryzae