Circ_0031027 adjusts the advancement of cervical cancer by miR-587/SOCS5 axis

Am J Reprod Immunol. 2022 Dec;88(6):e13640. doi: 10.1111/aji.13640. Epub 2022 Nov 7.

Abstract

Background: Circular RNAs (circRNAs) might participate in the growth of cervical cancer (CC). In this research, we reconnoitered the characters of circ_0031027 in CC.

Methods: Circ_0031027, microRNA-587 (miR-587), and suppressor of cytokine signaling 5 (SOCS5) abundances were distinguished by qRT-PCR and western blot. The cell functions were examined by colony formation assay, EdU assay, and transwell assay. The combined relationship of miR-587 and circ_0031027 or SOCS5 was identified by dual-luciferase reporter assay. Ultimately, the mice test was utilized to assess the part of circ_0031027.

Results: Circ_0031027 and SOCS5 were downregulated, and the miR-587 was upregulated in CC. For functional analysis, circ_0031027 overexpression repressed cell proliferation, cell migration, and invasion in CC cells. Circ_0031027 as a miR-587 sponge to adjust SOCS5. MiR-587 facilitated the advancement of CC cells by overturning SOCS5. In addition, circ_0031027 overexpression subdued tumor growth.

Conclusion: Circ_0031027 inhibited the enlargement of CC by miR-587/SOCS5.

Keywords: SOCS5; cervical cancer; circ_0031027; miR-587.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Female
  • Humans
  • Mice
  • MicroRNAs* / genetics
  • Signal Transduction
  • Suppressor of Cytokine Signaling Proteins / genetics
  • Uterine Cervical Neoplasms* / genetics

Substances

  • MicroRNAs
  • SOCS5 protein, human
  • Suppressor of Cytokine Signaling Proteins
  • MIRN587 microRNA, human