MXene as a novel cartridge for N-glycan enrichment

Juvissan Aguedo; Zuzana Pakanova; Lenka Lorencova; Marek Nemcovic; Peter Kasak; Marek Barath; Pavol Farkas; Jan Tkac

doi:10.1016/j.aca.2022.340512

MXene as a novel cartridge for N-glycan enrichment

Anal Chim Acta. 2022 Nov 22:1234:340512. doi: 10.1016/j.aca.2022.340512. Epub 2022 Oct 13.

Authors

Juvissan Aguedo¹, Zuzana Pakanova², Lenka Lorencova¹, Marek Nemcovic², Peter Kasak³, Marek Barath¹, Pavol Farkas¹, Jan Tkac⁴

Affiliations

¹ Institute of Chemistry, Slovak Academy of Sciences, 845 38, Bratislava, Slovakia.
² Centre of Excellence for Glycomic, Slovak Academy of Sciences, 845 38, Bratislava, Slovakia.
³ Center for Advances Materials, Qatar University, P. O. Box 2713, Doha, Qatar.
⁴ Institute of Chemistry, Slovak Academy of Sciences, 845 38, Bratislava, Slovakia. Electronic address: Jan.Tkac@savba.sk.

PMID: 36328727
DOI: 10.1016/j.aca.2022.340512

Abstract

In this study, we applied MXene as column cartridge for N-glycan enrichment from human samples with a focus on the analysis of sialic acid linkages using a derivatisation protocol followed by glycan analysis via Matrix Assisted Laser Desorption Ionisation-Time Of Flight Mass Spectrometry (MALDI-TOF-MS). The MXene-based cartridge enriches a higher number of glycans (i.e., sialylated and bisecting N-glycans) when compared to the commercial HILIC columns. We demonstrate the strong potential of MXene as a stationary phase in MS glycomic analysis.

Keywords: 2D nanomaterial; Glycan enrichment; Glycomics; Nanotechnology; Ti(3)C(2)T(x) MXene.

MeSH terms

Humans
N-Acetylneuraminic Acid*
Polysaccharides* / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Polysaccharides
N-Acetylneuraminic Acid