Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application

Kazunori Sugiura; Takeharu Nagai

doi:10.1038/s42003-022-04153-7

Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application

Commun Biol. 2022 Nov 3;5(1):1172. doi: 10.1038/s42003-022-04153-7.

Authors

Kazunori Sugiura¹, Takeharu Nagai²

Affiliations

¹ SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan.
² SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka, Japan. ng1@sanken.osaka-u.ac.jp.

Abstract

To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more than 10 years. Here, we report the development of Sumire, a fluorescent protein emitting 414 nm violet fluorescence from a hydrated chromophore. The Sumire's fluorescence property allows for the creation of FRET probes that can be used simultaneously with CFP-YFP based FRET probes for multi-parameter analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence Resonance Energy Transfer*
Green Fluorescent Proteins / metabolism
Luminescent Proteins / genetics
Luminescent Proteins / metabolism

Substances

Luminescent Proteins
Green Fluorescent Proteins