Ammonia nitrogen recovery from biogas slurry by SCP production using Candida utilis

Abstract

The safe and robust yeast Candida utilis was employed for nitrogen recovery as single cell protein from biogas slurry. The maximum biomass of 6.2 g/L with protein content of 53.5% was produced in batch cultivation with glucose as the carbon source, C/N ratio of 3:1, NH₄⁺-N concentration of 3000 mg/L, initial pH of 8.0, and the addition of 0.35% (w/v) Na₂HPO₄. It was speculated that C. utilis can grow well with free ammonia below 197 mg/L. In fed-batch fermentation, a biomass of 14.8 g/L was obtained, and the maintenance of aerobic conditions was critical to improving the production of single cell protein. The sterilized and non-sterilized biogas slurry can be used as an effective pH regulator. The obtained single cell protein was a nutritious, safe, and reliable protein source. This study provides novel insights into nitrogen recovery via C. utilis as a single cell protein from biogas slurry.

Keywords: Amino acids profile; Biogas slurry; Biomass accumulation; Candida utilis; Single cell protein.